Regulatory

Part:BBa_K5452107:Design

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-27)


Untranslated region located upstream to the S. cerevisiae hexokinase gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 278


Design Notes

We chose the hexokinase gene because its knockout does not interfere with yeast growth. Deletion of the HXK isoenzyme can trigger an increase in the expression of another HXK, causing an increase in the flow of the glycolytic pathway (Sun et al., 2014).


Source

This part can be found in the genome of the Saccharomyces cerevisiae

References